BioEnzyme Workshop conducted by GreenLine - Mumbai, India

On February 4, 2024, a workshop on making bioenzyme was conducted at Don Bosco Campus, Matunga, by GreenLine as part of the Green Lifestyle Initiative. The workshop focused on introducing participants to bioenzymes as sustainable alternatives to chemical household cleaning products, aiming to promote a greener future.


Bioenzymes are natural, eco-friendly substances produced through fermentation processes involving organic waste materials. They consist of beneficial bacteria and enzymes that break down organic matter, effectively cleaning and deodorizing surfaces. Unlike chemical cleaners, bioenzymes are non-toxic, biodegradable, and pose no harm to the environment.


Bioenzymes can replace conventional chemical cleaning agents in various household applications. They are effective in cleaning surfaces, removing stains, and eliminating odors.

Additionally, bioenzymes can be used in gardening as natural fertilizers and pest repellents. Their eco-friendly nature makes them ideal for individuals seeking sustainable lifestyle choices.


The workshop demonstrated a simple process for creating bioenzymes using citrus peels. Participants were guided through the following steps:

1. Participants were asked to get 300 gms of citrus peels (e.g., oranges, lemons) and chop

them into small pieces.

2. Place the citrus peels in a clean, airtight container (a plastic bottle of 2L), filling it up to

one-third of its capacity.

3. Fill one part of the container with jaggery.

4. Pour water (10 parts) into the container, leaving some space at the top for fermentation


5. Seal the container tightly and store it in a cool, dark place for three months, opening the lid of the container every day for a few seconds to release the gases.

6. After the fermentation period, strain the liquid from the container, which is now a

potent bioenzyme solution ready for use.


Participants actively engaged in the learning process. They showed a keen interest in understanding the science behind bioenzymes and their practical applications. Through

hands-on activities and demonstrations, attendees gained confidence in creating their own bioenzymes and incorporating them into their daily lives.


The workshop on making bioenzymes for the Green Lifestyle Initiative proved to be informative and engaging, creating awareness about sustainable alternatives to chemical household cleaning products. By promoting the use of bioenzymes, participants were empowered to make environmentally conscious choices, contributing to a greener and healthier future for all.